ABSTRACT
Objective: The aim of the present work was to develop and validate a simple UV spectroscopic method for the determination of duloxetine, which is a thiophene derivative and a selective neurotransmitter reuptake inhibitor for serotonin, norepinephrine, and to lesser degree dopamine. Methods: The UV Spectrophotometric analysis was performed using Shimadzu UV-1800 and Shimadzu UV-1700 spectrophotometer by using solvent system acetonitrile and water in the ratio of 8:2. Detection was performed at a wavelength of 290 nm. Method validation was carried out according to ICH Q2R1 guidelines by taking the parameters linearity, accuracy, precision, ruggedness, and robustness, LOD and LOQ. Results: The UV Spectrophotometric method was found linear in the range of 10-50 μg/ml. The method was rugged and robust with % relative standard deviation less than 2. The extraction recoveries were found to be higher than 99% in all experimental conditions. Conclusion: Based upon the performance characteristics, the proposed method was found accurate, precise and rapid and suitable for the determination of Duloxetine for routine analysis.
ABSTRACT
Cystic hygroma are benign tumors in the early childhood mainly manifesting in the head and neck region ..Cystic hygromas manifesting in elderly patients have not been reported in the literature so for. We report a rare presentation of cervical cystic hygroma in an adult male (58 yrs.), with sudden appearance and rapid progression to a relatively big size (10 cm x 12 cm) invloving superior mediastinum within a span of six months, giving a clue to malignant cystic pathology. Investigations proved the diagnosis of cystic hygroma. Transcervical excision was achieved with the help of cardiothoracic surgeons. The management of the cystic hygroma is being discussed.